Composite

Part:BBa_K1217009:Design

Designed by: Ma Tsz Shan   Group: iGEM13_Hong_Kong_HKU   (2013-09-15)


Localizing polyphosphate kinase (ppk1) into Histag modified Eut microcompartment (BBa_K311004)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2566
    Illegal NotI site found at 3476
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2779
    Illegal BglII site found at 3689
    Illegal XhoI site found at 2574
    Illegal XhoI site found at 3484
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3403
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2991
    Illegal SapI.rc site found at 366


Design Notes

Source

Kingella oralis (ATCC 51147), BBa_K311004

References